Originally Posted by
rpcrowe
Actually, I wanted to confirm or discount one of my thoughts about DOF. That thought is the depth of field in macro work is dependent on the image ratio and doesn't change with different focal lengths and camera to subject distances; as long as the image ratio remains the same.
In other words, an image with a 1:1 image ratio has the same DOF if it were shot with a 50mm, 60mm, 80mm, 100mm, 150mm or 180mm lens as long as the image was shot at the same f/stop and with the same format camera. The reason that the format would make a difference is that the circle of confusion for one format is different from a smaller or larger format.