I'm assuming you are referring to fitting extension tubes between camera and the lens Christina.
Fitting of extension tubes decreases the FoV that is recorded by the sensor. If we take your 105mm macro lens at it's minimum focus distance which equates to a magnification of 1:1 this simply translates that if you focus on a bug that is 23.6mm wide it will fill your sensor across its full width which is 23.6mm wide. Your working distance with this scenario, lens front to bug will be approx 150mm.
When we fit an extension tube it increases the lens original magnification but also reduces your working distance. So if we add a full set of tubes to your lens when it's at it's 1:1 magnification we just about double the magnification but reduce our working distance from 150mm down to 70mm approx. With double the magnification (now 2:1) you will only capture half of your 23.6mm wide bug on the sensor. The CiC tutorial covers the maths.
Any extension tube addition requires the camera/lens to subject distance to reduce thus reducing DoF. The affect of the reduction of DoF is perhaps not too significant as you subjects are also smaller but it can be compensated for by using a smaller aperture if lighting allows.
You are not the only one that finds it challenging to get a focussed shot of a bug that moves, we all do. As an example it took me about one hour to get the two spider shots, 5 shots taken, 3 straight to the bin, 1 50/50 and just two I was reasonably happy with.
As for tips with critters that move here's some of what I have learnt;
1 - General
a) The DoF you are able to achieve is going to be very dependent upon lighting available, flash is a great bonus. Do not worry too much about DoF an image of a fly with a brilliant sharp eye is just as impressive if a 1/4 or 3/4 of it's body is in focus.
b) Before shooting consider the bug size, how you want to fill the frame, magnification required. I tend to think that I want to fill 3/4 of the frame with the subject and aim not to crop later.
2 - Handholding
a) Do not waste time with live-view or mirror up.
b) Be pretty sure you are set up correctly before approaching the bug, take a test shot of something first as there's nothing worse than being focussed on that perfect pose in the frame pressing the shutter only to find you forgot to turn your flash on or left the camera set at ISO6400.
c) Preset you lens magnification first so that you do not have to put your hand on the lens barrel to rotate it scaring the bug.
d) Support yourself well and move in slowly concentrating on the framing firstly with the shutter half pressed.
e) As the subject becomes more in focus concentrate totally on the eye and only on the eye. The instant the eye is sharpest fully press the shutter.
3 - Tripod
a) With a bug that moves a lot this is real fun as the only option is to move the rig each time.
b) If it's not likely to stop in one position for very long forget mirror up.
c) Personally I never use live-view at all because I trust my focusing ability using the viewfinder.
d) I hold the remote release in my left hand, finger on button and as winding the focus ring (or at times my focus rail) the instant the eye is in focus snap the shot.
If your subjects are steady for long enough you have time to play with such things as aperture, lighting, mirror up e.t.c.
Hope there's something helpful here
Grahame