The following three shots, although technically not great, show what can happen when choosing Auto ISO while shooting in manual mode. The practice of 'floating' the ISO on cameras that provide the option, can be a wonderful time saver for sports and wildlife photography. However, like all things photographic, there is a compromise.
I discovered this for the first time just last month. I had been enjoying the benefit of my new 'trick' with ISO when I took this series of 3 shots in less than 1 second. I would never have thought that the camera would choose to change the ISO during three consecutive shots taken so rapidly.
As you can see, the 2nd and 3rd shots are at ISO 125, while the 1st shot is at ISO 100 and is noticeably darker.
I live and learn.............
1/1250, f/5.6, ISO 100
Myna Bath 1 by Chrisclick, on Flickr
1/1250, f/5.6, ISO 125
Myna Bath 2 by Chrisclick, on Flickr
1/1250, f/5.6, ISO 125
Myna Bath 3 by Chrisclick, on Flickr