Is there any method for estimating DOF when using extension tubes. For example I was photographing a butterfly egg with a 100 mm macro, and 65mm of extension tubes, (it was a very small egg). I tried stacking a number of images, but the best single shot was the best. I would have been close to the minimum focus of the lens. The minimum focus for the Canon 100mm IS macro is 300mm, and on a review does give the magnification for 10mm and 25mm extension rings, but not greater.