
Originally Posted by
DanK
I typically start with ISO 400 (again, fixed) for indoor flash work because with E-TTL flash and the camera in manual mode, it gives a reasonable balance between the flash and ambient light. I vary it as need be.
In macro with flash, I vary the ISO based on the brightness of the background (same logic as above) but generally stick with 100, 200, or 400.
If light is so low that I can't use the aperture and shutter speed I want with a low ISO, I raise it, but only as much as I need to, for the reasons, Manfred gave.
I use auto ISO only when the lighting is variable and low enough that I don't have wiggle room in terms of shutter speed and aperture.