I have been wondering which technique would be best for Focus Stacking, so I thought I would set up a trial with a couple of objects. The first is a Pine cone, about 7cm top-to-bottom and 5cm across. It was positioned with the tip of the cone facing the camera. The second was a sprig of Butcher's Broom Ruscus aculeatus, a strange member of the Lily family in which the leaves are replaced by woody expanded stems called cladodes. Currently the plant in my garden is bearing red berries just over 1cm across. The larger cladodes are about 2cm long.
All the images were processed using Helicon Focus (three algorithms) and Zerene Stacker (two algorithms). In all cases, the Zerene Depth Map have the best results.
#1 Pine Cone using lens focusing
#2 Pine Cone using a focusing rail
One thing that is apparent is that the lens-focussed image has some imperfections in alignment in the nearer parts of the cone, but it would be hard to tell any differences in the further parts. I think these imperfections were probably my fault.
#3 Butcher's Broom lens focusing
#4 Butcher's Broom focusing rail
One problem with the rail is that because of the camera angle, as I racked forward and back, the image drifted up and down. I had to re-align half way through. I fused the two final images from Zerene in Affinity.
I cannot see any real differences between the two. Given that, I would say that for most purposes, focusing using the lens would be the best bet. One problem with the lens focusing is that the total angle of rotation between front and back is quite small. I had to rig up a cardboard dial to gradually change the focusing position.
I would welcome any comment on these images as to which gives the best results.
John