A good subject for practicing with Brian
Some observations, comments and things to consider;
It appears from the caterpillar and this stack that your stacking software is working well so your main aim now is to perfect your procedure. That means confidently determining your required near and farthest focus planes (points is ok) and then deciding your procedure to divide and adjust this into the number of divisions you want equally.
This may mean 'marking' your focus barrel in some way (tape?) at the two extremes (which we would normally just go past), or noting distance scale readings. Then comes the tricky part, how do you divide this angular distance into say 25 divisions?
On my lens the rotating focus barrel has long straight longitudinal serrations and a white spot on the non-rotating part adjacent to these and it's simply a matter of counting the number of serrations (ridges) moved each time.
Good luck