Originally Posted by
Manfred M
Andre - I'm not an expert here, but your focusing method might be causing some of the issue.
All lenses exhibit something called "focus breathing", where the image size changes slightly as you change focus on the lens. In most day-to-day photography, we never notice that, but it places where we are trying for in-shot focus changes, it can make a difference. My own experience relates to cinematography where focus is sometimes pulled in a scene; for instance focusing on an object in the scene and then, without changing camera position or focal length, focusing on something else in the scene.
In closueup work like you are doing, this can impact the image. My understanding is that is why advanced macro shooters use a focus rail to move the entire camera when doing focus stacking. This also seems to be the case where in landscape photography, focus stacking is becoming very much in fashion. I'm thinking of getting a focusing rail (rather than using my long rail) to avoid the issue you are seeing in my landscape work.