I did this focus stack using twenty images shot with my Sony 7Diii and Venus Optic 60mm f/2.8 2:1 Macro Lens. The camera/lens was on a focus rail and I shot the images at f/8. I changed the focus plane with the wheel of the focus rail with the front of te hook in focus for the first image. The wheel was turned approximately the same for each shot... I used a 3-second delay in shooting each image.
I opened the images using Adobe Bridge and Adobe Camera RAW and did stacking in Photoshop 2020.
The Wooly Bugger fly is a bit over 3-inches (abt 75mm) long.
Everything looks O.K. except for the area around the barb of the hook...
My Photoshop 2020 stacking process was this:
1. FILE>scripts>load files into stack
2. SELECT>all layers
3. EDIT>auto blend layers>stack images>seamless tones and colors>content aware fill transparent areas
What might the trouble be?
I did this focus stack of an inch and a half long (abt. 40mm) Royal Coachman Streamer at f/11 with 9-images. I eyeballed the focus as I rotated the wheel of the focus rail using focus peaking...
There doesn't seem to be a problem with this one...